Typical Functional Requirements for HPLC with UV . Vis Detector Modules Functional Requirements

Typical Functional Requirements for HPLC with UV . Vis Detector
Modules Functional Requirements
Pump . The pump should be capable of a fl ow rate between 0.50
and 5.00 mL/min.
. The pump should be a quaternary gradient pump and have a
compositional accuracy of } 1.5% of the theoretical values for
the four channels.
. The pump should have relative standard deviation (RSD)
of . 2.0% for six successive readings from a calibrated
fl owmeter.
Autosampler . The autosampler should have an injector capable of injecting
sample volumes from 1 to 100 ƒÊ L.
. The injector should have a precision of . 1.5% RSD.
. The injector carryover should be . 0.5% of peak area.
. The autosampler should pick the correct vial.
. The autosampler should use relays and/or contacts to
communicate with the laboratory CDS.
. Temperature - controlled sample racks should be capable of
maintaining samples in the temperature range of 4 . 15 ‹ C
(} 3 ‹ C).
UV . Vis detector . The detectors should operate with a wavelength range from
200 to 800 nm.
. The detector should have a wavelength accuracy of } 2 nm.
. The detector response should be linear with a correlation
coeffi cient r2 not less than 0.999 over the full dynamic range.
. The linear range should be up to 2.0 Absorbance Unit Full
Scale (AUFS) .
Column compartment . The column oven should be capable of maintaining a
temperature range of 5 ‹ C above ambient to 60 ‹ C.
. The oven should be within } 3 ‹ C of the set temperature and
the temperature precision should be . 2.0%.