HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
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